A gel filtration method for studies on protein iron-binding
β Scribed by Albert A. Barber; Carol Dempster; Norman G. Anderson
- Book ID
- 115816731
- Publisher
- Elsevier Science
- Year
- 1963
- Tongue
- English
- Weight
- 222 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0009-8981
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π SIMILAR VOLUMES
A double conical gel-permeation column is described, which permits an efficient dispersion of solutes in passage. It makes possible a facile determination of ligand binding to protein on the basis of the differential gel permeation of these molecules. The system has been applied, using Sephadex G-25
A new method for specifically staining the iron atoms present in transfenin and lactoferrin after polyacrylamide gel electrophoresis and isoelectric focusing is described. The stain, 3-(2pyridyl)-5,6-bis(2-(5-furylsulfonic acid))-1,2,4-triazine, disodium salt, or Ferene S, will detect transferrin in