A forced-flow membrane reactor for transfructosylation using ceramic membrane

✍ Scribed by Koji Nishizawa; Mitsutoshi Nakajima; Hiroshi Nabetani

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 148 KB
• Volume: 68
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(20000405)68:1<92::aid-bit11>3.0.co;2-1

✦ Synopsis

A forced-flow membrane reactor system for transfructosylation was investigated using several ceramic membranes having different pore sizes. beta-Fructofuranosidase from Aspergillus niger ATCC 20611 was immobilized chemically to the inner surface of a ceramic membrane activated by a silane-coupling reagent. Sucrose solution was forced through the ceramic membrane by crossflow filtration while transfructosylation took place. The saccharide composition of the product, which was a mixture of fructooligosaccharides (FOS), was a function of the permeate flux, which was easily controlled by pressure. Using 0.2 micrometer pore size of symmetric ceramic membrane, the volumetric productivity obtained was 3.87 kg m(-3) s(-1), which was 560 times higher than that in a reported batch system, with a short residence time of 11 s. The half-life of the immobilized enzyme in the membrane was estimated to be 35 days by a long-term operation.