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A fluorometric assay to monitor mitogenic stimulation of human lymphocytes

✍ Scribed by Ralf Bialek; Hinrich Abken

Book ID
118902198
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
457 KB
Volume
144
Category
Article
ISSN
0022-1759

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Proliferation of human lymphocytes in response to various stimuli has traditionally been assessed by measuring uptake of radiolabeled nucleotides such as3H-thymidine. We have evaluated a fluorometric assay, which uses the commercially available reagent, alamarBlue, as a potential substitute for the3