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A fluorometric assay for β-phenylethylamine in rat brain

✍ Scribed by Osamu Suzuki; Kunio Yagi

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
497 KB
Volume
75
Category
Article
ISSN
0003-2697

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✦ Synopsis

The amount of /3-phenylethylamine in rat brain could be determined by this reaction after its separation from phenylalanine and Shydroxytryptamine by n-heptane extraction. The content of endogenous Pphenylethylamine in rat brain was 5 rig/g of wet tissue. Administration of pargyline caused more than a IO-fold increase in brain pphenylethylamine.

After the injection of both pargyline and phenylalanine, /3-phenylethylamine was increased about lOO-fold. This increase was reduced by prior injection of reserpine, while enhanced by that of calcium fusarate.

MATERIALS

PEA-HCl

(Nakarai Chemicals Co., Kyoto) was recrystallized three times from ethanol by the addition of acetone, and the crystals were 192 Copyright

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