๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A Fluorometric Assay for Measuring Deamidase (Lysosomal Protective Protein) Using High-Performance Liquid Chromatography

โœ Scribed by Toshiyuki Chikuma; Kayano Matsumoto; Atsuko Furukawa; Noriko Nakayama; Ryuichi Yajima; Takeshi Kato; Yoko Ishii; Akira Tanaka


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
94 KB
Volume
233
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


A fluorometric assay for HIV-protease ac
โœ Paul P. Tamburini; Robert N. Dreyer; Jutta Hansen; Jack Letsinger; Jim Elting; A ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 883 KB

A rapid sensitive method for the quantification of in vitro HIV-protease activity has been developed on the basis of the endoproteolytic conversion of N-Dns-SQ-NYPIV to N-Dns-SQNY. The use of the N-dansyl group as a fluorescence label was shown to not significantly alter the apparent kinetic paramet

A fluorometric assay for peptidyl ฮฑ-amid
โœ Barry N. Jones; Paul P. Tamburini; Angelo P. Consalvo; Stanley D. Young; Susan J ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 663 KB

A rapid and sensitive method for the determination of peptidyl alpha-amidation activity has been developed and is based on reverse-phase high-performance liquid chromatographic separation and fluorometric detection. A dansylated tripeptide, N-dansyl-Tyr-Val-Gly-OH, is used as the substrate in the as

A rapid fluorometric assay for N-termina
โœ Angelo P. Consalvo; Stanley D. Young; Barry N. Jones; Paul P. Tamburini ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 713 KB

A rapid, sensitive method for the quantification of glutaminyl cyclase activity has been developed. The assay involves enzymatic conversion of the model peptide Gln-Leu-Tyr-Glu-Asn-Lys-epsilon-(Dns)-OH to less than Glu-Leu-Tyr-Glu-Asn-Lys-epsilon-(Dns)-OH. Both the product and substrate of this reac

A colorimetric assay for measuring pepti
โœ Toshiyuki Chikuma; Ko-ichi Hanaoka; Y. Peng Loh; Takeshi Kato; Yoko Ishii ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 595 KB

In many peptide hormones and neuropeptides, the carboxy-terminal alpha-amide structure is essential in eliciting biological activity. In the present study, a rapid and sensitive assay method for the determination of peptidylglycine alpha-amidating monooxygenase (PAM) activity has been reported. This

Measurement of Nitric Oxide Metabolites
โœ Johannes Woitzik; Nils Abromeit; Franz Schaefer ๐Ÿ“‚ Article ๐Ÿ“… 2001 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 148 KB

Nitric oxide (NO), formed from arginine by a specific neuronal NO synthase, is an important neurotransmitter in various regions of the central nervous system. While intracerebral microdialysis is an elegant technique to study local extracellular neurotransmitter concentrations in vivo, NO metabolite

A fluorometric method for measuring etho
โœ Daniel W. Rosenberg; Henry Roque; Attallah Kappas ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 582 KB

A high-performance liquid chromatographic (HPLC) method that uses an on-line change in the protonation state of the nonfluorescent product to yield a fluorescent derivative that is detected by fluorometry was developed for the determination of 7-ethoxycoumarin O-deethylase activity. Tissue samples (