A fluorogenic, mixed-disulfide reagent for thiol assay

✍ Scribed by Kevin J. Willis; Francis W.J. Teale

• Book ID: 102984966
• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 990 KB
• Volume: 153
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(86)90101-6

✦ Synopsis

The design and synthesis of the mixed disulfide (ortho-nitroaniline-N-ethyldithio) 2-[pyridyl-5-thioureido-N'-(5-fluorescein)] are described and the chemical and spectroscopic properties of this thiol-specific fluorogenic reagent are presented. The high reactivity and sensitivity of this reagent in thiol assay are demonstrated with low-molecular-weight thiols and with human carbonmonoxyhemoglobin and its subunits. Comparison with conventional absorption methods shows that at least 100 times less material is needed; moreover, high background absorbance or turbidity do not interfere with the assay.