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A fluorimetric method for determination of calcineurin activity

✍ Scribed by Brian Roberts; Jan Pohl; Jennifer L. Gooch

Book ID
113525729
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
416 KB
Volume
43
Category
Article
ISSN
0143-4160

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A sensitive fluorimetric method with guaiacol as the hydrogen donor for peroxidase activity is described. The gist of this method is measurement of the fluorescence (excitation, 300 nm; emission, 340 nm) in cyclohexane of mainly a guaiacol dimer which forms in the early phase of the color formation.