Although Rinderknecht (9) proposed the use of DANS-Cl adsorbed on Celite to avoid the employment of organic solvent, the reaction was still heterogeneous and the degree of labeling (D. L.) was often poor '(5). This paper deals with the preparation of the complex of DANS-Cl with cycloheptaamylose (C
A fluorescent label for the outer components of the plasma membrane
β Scribed by A.H. Maddy
- Publisher
- Elsevier Science
- Year
- 1964
- Weight
- 576 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0926-6577
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A method, using albumin-pyrene complexes, has been developed for labeling, in a controlled manner, crab leg nerves whose excitability was preserved. The excimer-to-monomer fluorescence intensity ratio of pyrene, embedded in nerve membrane lipids and in their crude lipid extracts, is a fluidity param
Three different fluorochromes were tested for their ability to label the plasma membrane proteins of neutrophils without labelling intracellular structures. A fluorescence quenching technique was used to differentiate between extra- and intracellularly localized fluorescence. Fluorescamin and fluore