A FLUORESCENCE STUDY OF THE BINDING OF HOECHST 33258 and DAPI TO HALOGENATED DNAs
โ Scribed by Torleif Hard; Pei Fan; David R. Kearns
- Book ID
- 114895730
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 860 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0031-8655
No coin nor oath required. For personal study only.
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Application of the fluorescent DNA-intercalator propidium iodide for stabilization of the mitotic chromosome structure during isolation of chromosomes from V79 Chinese hamster cells and subsequent staining with the fluorochromes 33258 Hoechst or DAPI allowed bivariate flow karyotyping of isolated ch
## Abstract The groove binding dye Hoechst 33258 was investigated both in aqueous solution and in the adsorbed form on silver colloids and island films. Fourier transform Raman, resonance Raman and surface enhanced resonance Raman spectra were used together with the results of a normal coordinate a