A fluorescence lifetime-based fibre-optic glucose sensor using glucose/galactose-binding protein
โ Scribed by Saxl, Tania ;Khan, Faaizah ;Ferla, Matteo ;Birch, David ;Pickup, John
- Book ID
- 120176717
- Publisher
- Royal Society of Chemistry
- Year
- 2011
- Tongue
- English
- Weight
- 225 KB
- Volume
- 136
- Category
- Article
- ISSN
- 0003-2654
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๐ SIMILAR VOLUMES
We describe a glucose sensor based on a mutant glucose/galactose binding protein (GGBP) and phase-modulation fluorometry. The GGBP from Escherichia coli was mutated to contain a single cysteine residue at position 26. When labeled with a sulfhydryl-reactive probe 2-(4'-iodoacetamidoanilino)naphthale
The galactose/glucose-binding protein (GBP) is synthesized in the cytoplasm of Escherichia coli in a precursor form and exported into the periplasmic space upon cleavage of a 23-amino-acid leader sequence. GBP binds galactose and glucose in a highly specific manner. The ligand induces a hinge motion