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A fluorescence correlation spectroscopy-based enzyme assay for human Dicer

✍ Scribed by Werner, Arne; Skakun, Victor V.; Ziegelmüller, Patrick; Hahn, Ulrich

Book ID
115542620
Publisher
Walter de Gruyter GmbH & Co. KG
Year
2012
Tongue
English
Weight
476 KB
Volume
393
Category
Article
ISSN
1431-6730

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A fluorescence correlation spectroscopy-
A fluorescence correlation spectroscopy-based enzyme assay for human Dicer
✍ Werner, Arne; Skakun, Victor V.; Ziegelmüller, Patrick; Hahn, Ulrich 📂 Article 📅 2012 🏛 Walter de Gruyter GmbH & Co. KG 🌐 English ⚖ 476 KB

## Abstract Here, we present an __in vitro__ assay based on fluorescence correlation spectroscopy (FCS), which allows investigation of the kinetic behaviour of human Dicer. The assay is based on the different mobilities of substrate and product. The change of substrate mobility was independent of t

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