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A fluorescence assay for phenylalanine transfer RNA

✍ Scribed by Doju Yoshikami; Gerson Katz; Elizabeth B. Keller; Bernard S. Dudock


Book ID
115743246
Publisher
Elsevier Science
Year
1968
Weight
259 KB
Volume
166
Category
Article
ISSN
0005-2787

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A continuous spectrophotometric assay is described for the aminoacylation reaction catalyzed by Escherichia coli alanyl-transfer RNA synthetase. The assay is based on coupling the alanyl-tRNA synthetase-dependent formation of AMP to the lactate dehydrogenase oxidation of NADH. Oxidation of NADH, as