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A Fluorescamine-Based Sensitive Method for the Assay of Proteinases, Capable of Detecting the Initial Cleavage Steps of a Protein

✍ Scribed by Rafael GARESSE; José V. CASTELL; Carmen G. VALLEJO; Roberto MARCO

Book ID
115118513
Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
564 KB
Volume
99
Category
Article
ISSN
1432-1327

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A 96-well microtiter enzyme-linked immunosorbent assay (ELISA) for protein tyrosine kinases has been developed. This assay uses one of several substrates that are phosphorylated by tyrosine kinase, an antibody to phosphotyrosine, and a peroxidase-linked second antibody. Color development is monitore