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A flow cytometric method for the detection of the development of antibody to Orthoclone OKT3

โœ Scribed by Victor L. Lim; Marianne Gumbert; Marvin R. Garovoy


Book ID
118901500
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
377 KB
Volume
121
Category
Article
ISSN
0022-1759

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Intracellular glycosyltransferase protein expression can be assessed by flow cytometry. We report the detectability of the Golgi associated b1,4 galactosyltransferase (GT) and a2,6 sialyltransferase (ST) upon permeabilization in Jurkat and EBV-JY cells representing a T-and B-lymphoid cell line, resp