A filter affinity transfer method for the analysis of rheumatoid factors

✍ Scribed by Pierre Aucouturier; Jean-Paul Maillochon; Guy Joseph-Théodore; Francoise Duarte; Jean-Louis Preud'Homme

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 383 KB
• Volume: 5
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860050603

✦ Synopsis

Transfer of serum proteins separated by thin layer agarose electrophoresis onto nitrocellulose sheets precoated with purified human polyclonal IgG followed by revelation with enzyme-coupled anti-p, or anti-cY antisera resulted in the specific detection of rheumatoid factors (RF) belonging to the IgM or IgA classes. Mono-or polyclonality of such RF can be evaluated from the patterns of the blots (sharp bands). In addition, their light chain type can be determined using affinity filters coated with a y heavy chain disease protein or with IgG Fc fragments. This simple and rapid procedure allows an easy characterization of monoclonal RF, even if they are present in minute amount amongst polyclonal RF as in certain sera from rheumatoid arthritis patients.