A fiber-bed bioreactor for anchorage-dependent animal cell cultures: Part II. Scaleup potential

✍ Scribed by Sei Murakami; Tzyy-Wen Chiou; Daniel I. C. Wang

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 641 KB
• Volume: 37
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260370811

✦ Synopsis

A simple hydrodynamic model is introduced to describe the airlift fiber-bed bioreactor, which can enhance the volumetric productivity of anchorage-dependent animal cell cultures. By applying the model, liquid flow rates and volumetric mass transfer coefficients are predicted and are in agreement with experimental measurements. Consequently, the optimal reactor configuration giving the maximal oxygen supply is derived. Also, theoretical scaleup potential of this concentric internal loop reactor is considered for volumes ranging from 10 to 67,000 L with which cell densities of 5.1 x lo7 and 1.2 x lo7 cells/cm3, respectively, can be maintained.

Key words: animal cell culturebioreactor airlift fiber-bed bioreactoroxygen transfer * To whom all correspondencc should be addressed.