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A fast, simple and sensitive method for the detection and quantification of detergent-resistant membranes

✍ Scribed by Norbert Blank; Christoph Gabler; Martin Schiller; Martin Kriegel; Joachim R Kalden; Hanns-Martin Lorenz

Book ID
114051173
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
249 KB
Volume
271
Category
Article
ISSN
0022-1759

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We describe a sensitive new method for specific detection and quantitation of phospholipid-binding proteins using two purified lipocortins as model proteins. The method consists of blotting proteins to nitrocellulose membranes followed by incubation with radiolabeled phospholipids and autoradiograph