✦ LIBER ✦

A fast screening method for the identification of siderophores from fluorescentPseudomonas spp. by liquid chromatography/electrospray mass spectrometry

✍ Scribed by Kilz, S.; Lenz, Ch.; Fuchs, R.; Budzikiewicz, H.

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 240 KB
Volume: 34
Category: Article
ISSN: 1076-5174
DOI: 10.1002/(sici)1096-9888(199904)34:4<281::aid-jms750>3.0.co;2-m

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✦ Synopsis

A screening method was developed for the fast identiÐcation of known pyoverdin-type siderophores produced by Ñuorescent Pseudomonas spp. It is based on reversed-phase high-performance liquid chromatography interfaced with electrospray ionization mass spectrometry of the Sep-Pak culture supernatant extracts. The side-RP-C 18 rophores of Ðve bacterial strains were characterized by their molecular masses obtained from their doubly protonated molecular ions [ M + 2H ] 2' and their UV/visible spectra recorded with a diode-array detector. Additional structural information was gained by skimmer collision-induced dissociation experiments. For all strains new minor siderophores were found. A table of fully or partially identiÐed pyoverdins and related siderophores is provided which will be the basis for screening studies.

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