A fast and sensitive 1H NMR method to me
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Tiago B. Rodrigues; SebastiΓ‘n CerdΓ‘n
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Article
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2005
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John Wiley and Sons
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English
β 309 KB
## Abstract A fast and sensitive procedure to determine the turnover of the H2 hydrogen of lactate and quantify its ^2^Hβenrichment by ^1^H NMR is illustrated using C6 cells metabolizing (3β^13^C) lactate in 50% ^2^H~2~O (vol/vol). ^2^H substitution of the lactate H2 hydrogen resulted in two easily