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A Family 2a Carbohydrate-Binding Module Suitable as an Affinity Tag for Proteins Produced in Pichia pastoris

✍ Scribed by Alisdair B. Boraston; Bradley W. McLean; Marta M. Guarna; Emily Amandaron-Akow; Douglas G. Kilburn

Book ID
115645810
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
197 KB
Volume
21
Category
Article
ISSN
1046-5928

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Choline-binding domain as a novel affini
Choline-binding domain as a novel affinity tag for purification of fusion proteins produced in Pichia pastoris
✍ J. Caubín; H. Martín; A. Roa; I. Cosano; M. Pozuelo; J. M. de la Fuente; J. M. S 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 511 KB

## Abstract The choline‐binding domain (ChoBD) of the carboxy‐terminal region of the __Streptococcus pneumoniae__ amidase LYTA (C‐LYTA) presents a strong affinity for tertiary amines. We report a method for single‐step purification of proteins expressed in the methylotrophic yeast __Pichia pastoris