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A facile synthesis of δ-aminolevulinic acid (ALA) regioselectively labeled with 13C and direct observation of enzymatic transformation from ALA to porphobilinogen (PBG)

✍ Scribed by Katsuyuki Kurumaya; Takeo Okazaki; Nobuo Seido; Yuzuru Akasaka; Yoshiki Kawajiri; Masahiro Kajiwara; Masao Kondo


Publisher
John Wiley and Sons
Year
1989
Tongue
French
Weight
656 KB
Volume
27
Category
Article
ISSN
0022-2135

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✦ Synopsis


6-Aminolevulinic acid (ALA), labeled with 3C at position 1 , 2, 3, 4 , or 5, was synthesized from 13C-labeled glycine, Meldrum's acid, or bromoacetate. The latter compounds were prepared from 3C-sodium acetate or 3C-acetic acid.

Enzymatic transformation from ALA to porphobilinogen (PBG) was directly observed by 3C-NMR.