Screening of a representative series of immobilized penicillin G acylase biocatalysts (enzyme, cells) using enzyme Ñow microcalorimetry is described. Immobilized penicillin G acylase biocatalysts were either prepared in the laboratory by various techniques or obtained from four commercial manufactur
✦ LIBER ✦
A facile pretreatment method for efficient immobilization of penicillin G acylase
✍ Scribed by Pixiang Wang; Xiangyu Gong; Erzheng Su; Jingli Xie; Dongzhi Wei
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 419 KB
- Volume
- 56
- Category
- Article
- ISSN
- 1369-703X
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The possibility of using the multicompartment immobilized enzyme reactor (MIER) in presence of a charged substrate is here explored. Penicillin G acylase is used to convert penicillin G (a free acid, with a pK of 2.6) into two charged products: phenyl acetic acid (PAA, with a pK of 4.2) and 6-aminop
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