A facile method for detection of the genuine sapogenin from a saponin extract

✍ Scribed by R. Segal; I. Milo-Goldzweig; D.V. Zaitschek; M. Noam

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 430 KB
• Volume: 84
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90485-2

✦ Synopsis

A new method by which the authentic hemolytically active steroidal sapogenins can be detected by tic is described. The method is based on previous findings that saponin-induced hemolysis is preceded by hydrolysis of the glycosidic bonds. In this method the aqueous plant extract is incubated with bovine erythrocytes, the ghost cells of the hemdysed blood are collected, and the sapogenin is extracted from them with chloroform. When the extract is chromatographed (tic) and sprayed with a specific spray reagent, the steroidal sapogenins can be easily and unequivocally recognised.