✦ LIBER ✦

A Dye Binding Assay for the Quantification of Soluble and Cell-Bound Acidic Polysaccharides Produced by Red Algae

✍ Scribed by Ellen M. Hoffmann; Nicole Bauknecht

Publisher: Elsevier Science
Year: 1999
Tongue: English
Weight: 85 KB
Volume: 267
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1998.2976

No coin nor oath required. For personal study only.

✦ Synopsis

Polysaccharides produced in technical scale from red algae serve in a variety of industrial applications. Ruthenium red (RR) is a cytochemical stain which has been used to visualize these mostly acidic polysaccharides in light microscopy. The binding of RR to algal polysaccharides is highly dependent on the ionic strength of the surrounding medium. The dye is firmly bound at low ionic strength, but is released at increasing salt concentrations. These properties were exploited to develop a new method to quantify the amount of cell wall material synthesized by algal cells under different physiological conditions. Soluble or extracted polysaccharides are quantified by a dot-blot procedure with subsequent image analysis, while cellbound polysaccharides are determined in situ by a dye-binding assay followed by photometry. In comparison to the current methods of quantification, the new assay is quick, reliable, and avoids extensive use of hazardous chemicals.

📜 SIMILAR VOLUMES

Detection and quantification of CD8+ T c

Detection and quantification of CD8+ T cells specific for HLA-A*0201-binding melanoma and viral peptides by the IFN-γ-elispot assay

✍ Marieke Griffioen; Martina Borghi; Peter I. Schrier; Susanne Osanto 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 French ⚖ 122 KB 👁 1 views

Blood lymphocytes from HLA-A\*0201-subtyped melanoma patients and healthy controls were screened for the presence of T cells specific for HLA-A\*0201-binding melanoma and viral peptide antigens by the enzyme-linked immunoSPOT (ELISPOT) assay. CD8(+) cells were tested for peptide-specific IFN-gamma r