A dual function activity-dependent, muscle-specific enhancer from rat nicotinic acetylcholine receptor ?-subunit gene
✍ Scribed by Walke, Wade ;Xiao, Guozhi ;Goldman, Daniel
- Book ID
- 102655482
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 971 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
Nicotinic acetylcholine receptors (nAChR) mediate communication between nerve and muscle. The expression of these receptors increases dramatically during muscle development when myoblasts are fusing into multinucleated myotubes. The molecular mechanisms mediating this muscle developmental stage specific expression are not well understood. W e report here the identification of nAChR b-subunit promoter DNA sequences that differentially interact with nuclear proteins isolated from myoblasts, myotubes, and nonmuscle cells. The functional role these sequences play in mediating musclespecific expression was explored using mutagenesis and enhancer assays. These studies resulted in the identifi-