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A dual expression platform to optimize the soluble production of heterologous proteins in the periplasm ofEscherichia coli

✍ Scribed by Mario Kraft; Uwe Knüpfer; Rolf Wenderoth; André Kacholdt; Patricia Pietschmann; Björn Hock; Uwe Horn


Publisher
Springer
Year
2007
Tongue
English
Weight
431 KB
Volume
76
Category
Article
ISSN
1432-0614

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✍ François Baneyx; Amanda Ayling; Terry Palumbo; Daniel Thomas; George Georgiou 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 861 KB

The expression of many secreted recombinant proteins in Gram-negative bacteria is limited by degradation in the periplasmic space. We have previously shown that the production of protein A-fl-lactamase, a secreted fusion protein highly sensitive to proteolysis in Escherichia coli, can be increased i