Optimization of growth conditions for th
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François Baneyx; Amanda Ayling; Terry Palumbo; Daniel Thomas; George Georgiou
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Article
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1991
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Springer
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English
⚖ 861 KB
The expression of many secreted recombinant proteins in Gram-negative bacteria is limited by degradation in the periplasmic space. We have previously shown that the production of protein A-fl-lactamase, a secreted fusion protein highly sensitive to proteolysis in Escherichia coli, can be increased i