✦ LIBER ✦

A double-sandwich ELISA test for Glm(3) phenotyping of bloodstains with a mouse monoclonal antibody

✍ Scribed by Ch. Francois-Gerard; B. Hoste

Book ID: 104119576
Publisher: Elsevier
Year: 1987
Tongue: English
Weight: 973 KB
Volume: 27
Category: Article
ISSN: 0015-7368
DOI: 10.1016/s0015-7368(87)72704-2

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✦ Synopsis

ELISA procedures have been developed for the Gm(3) allotypic marker of human IgGl immunoglobulins, using a mouse monoclonal anti-Gm(3) antibody. The test has a high sensitivity since the monoclonal reagent, even diluted 11 32,000, still detects the Gm(3) antigen in serum samples diluted 1/128,000 to 1/2,000,000. On account of its sensitivity and its objective reading, the test is well adapted for bloodstain typing. The addition of a parallel test for IgG makes it possible to distinguish negative results due to Gm(-3) phenotypes from those due to a lack of IgG material. Gm(3) and IgG were detected in bloodstains more than 5 years old.

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