Implementation of a Continuous, Enzyme-C
โ
Kathryn E. McElroy; Peter J. Bouchard; Mark R. Harpel; Kurumi Y. Horiuchi; Kelle
๐
Article
๐
2000
๐
Elsevier Science
๐
English
โ 77 KB
Enzymatic formation of glutamate is critical to numerous biological pathways. However, current methods for assaying the activities of glutamate-forming enzymes are not particularly suitable for highthroughput screening in drug discovery. We present a continuous-read, fluorometric assay for high-thro