A dot-elisa intended for the specific and simultaneous detection of antibodies directed to antigens derived from toxoplasma gondii, rubella virus, cytomegalovirus, and type 1 and type 2 herpesviruses
✍ Scribed by Maria S. Leonardi; Sebastiana Zummo; Pasquale Mastroeni; Michéle Fattal-German; Bernard Bizzini
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 631 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0887-8013
No coin nor oath required. For personal study only.
✦ Synopsis
A procedure for the routine and simultaneous laboratory detection of IgG antibodies produced in humans in the course of various infectious diseases is described. The procedure, based on dot-enzyme-linked immunosorbent assay (ELISA), used single nitrocellulose strips onto which several antigens were dotted in close proximity. Optimal conditions were specified that allowed the unequivocal and simultaneous detection of IgG antibodies specifically directed against Toxopfasma gondii, rubella virus, cytomegalovirus, and herpes simplex virus type 1 and type 2 antigens. This technique has proved to be simultaneously specific, sensitive, and reliable, and it has been applied to prenatal screening of sera from pregnant women. It is suggested that this technique should also be used for the screening of large numbers of sera under field trial conditions.