A dominant selectable marker that is meiotically stable inNeurospora crassa: theamdSgene ofAspergillus nidulans
✍ Scribed by Carl T. Yamashiro; Oded Yarden; Charles Yanofsky
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 565 KB
- Volume
- 236
- Category
- Article
- ISSN
- 1617-4615
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✦ Synopsis
When Neurospora crassa is transformed using a Neurospora gene as the selectable marker, the vegetatively stable transformants obtained cannot be used successfully in a cross because the selectable marker will be inactivated by the process of RIP (repeat-induced point mutation). Introduction of the acetamidase-encoding gene amdS of Aspergillus nidulans into N. crassa by transformation yielded transformants that would grow in minimal medium containing acetamide as a sole nitrogen source. In mitotically stable transformants containing a single copy of the amdS gene, the capacity to utilize acetamide as a sole nitrogen source was maintained in the progeny of a sexual cross. Therefore, the A. nidulans amdS gene is an appropriate dominant selectable marker for use in transformation analyses with N. crassa in which sexual crosses will be subsequently performed.