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A DNA extraction method of small quantities of bone for high-quality genotyping

✍ Scribed by Caputo, Mariela; Irisarri, Maximiliano; Alechine, Evguenia; Corach, Daniel

Book ID
120556724
Publisher
Elsevier
Year
2013
Tongue
English
Weight
760 KB
Volume
7
Category
Article
ISSN
1872-4973

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In this study, we report a modified procedure for extraction of high-quality genomic DNA that is rapid, simple, biologically nonhazardous, and generally applicable to pathogenic bacteria. Bacterial cells were pretreated with 70% ethanol prior to enzymatic digestion with lysozyme. Exposure of bacteri