✦ LIBER ✦

A direct assay for collagen synthesis based on the estimation of collagenase-derived N-glycyl peptides

✍ Scribed by Charles Lynch; Carolyn Lara-Braud; Thomas Conway

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 647 KB
Volume: 83
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90511-5

No coin nor oath required. For personal study only.

✦ Synopsis

The synthesis of glycine-labeled collagen can be followed by a sensitive assay that detects radioactivity in glycine phenylthiohydantoin produced from N-glycyl peptides generated by clostridial collagenase. The assay is sensitive and highly specific for the direct measurement of collagen synthesis. Many commonly used assays are indirect, in that they measure the difference in TCA-precipitable radioactivity before and after collagenase treatment. In addition, the new assay is less affected by nonspecific proteases, which contaminate many commercial collagenase preparations and lead to erroneously high estimates of collagen by the indirect assay.

📜 SIMILAR VOLUMES

Synthesis and utilization of a novel gly

Synthesis and utilization of a novel glycine derived chiral precursor, based on a recyclable L-Prolinol auxiliary, for the enantioselective preparation of α-amino acids and their N-methyl derivatives

✍ Ganesh Pandey; P.Yella Reddy; Parthasarathi Das 📂 Article 📅 1996 🏛 Elsevier Science 🌐 French ⚖ 194 KB

ChemInform Abstract: A New Strategy for

ChemInform Abstract: A New Strategy for the Synthesis of 1,4-Benzodiazepine Derivatives Based on the Tandem N-Alkylation—Ring Opening—Cyclization Reactions of Methyl 1-Arylaziridine-2-carboxylates with N-[2-Bromomethyl(phenyl)]trifluoroacetamides.

✍ Jin-Yuan Wang; Xue-Fei Guo; De-Xiang Wang; Zhi-Tang Huang; Mei-Xiang Wang 📂 Article 📅 2008 🏛 John Wiley and Sons ⚖ 23 KB 👁 2 views

## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable v