𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A diffusion model and optimal cell loading for immobilized cell biocatalysts

✍ Scribed by Yujun Zhao; George B. Delancey

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
177 KB
Volume
69
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

✦ Synopsis

A diffusion model based on the random pore model is derived for immobilized cell biocatalysts and verified with 19 sets of experimental diffusion data. The predicted effective diffusivity relative to that for the support matrix reflects a quadratic dependence on the cell loading and contains a single parameter that depends on the intracellular diffusivity and the chemical partitioning coefficient. The model is used to predict optimal cell loadings that maximize the total reaction rate in an immobilized cell biocatalyst. A rule of thumb based on the diffusion model is obtained to the effect that the cell loading should be at least (1/3) for single reactions regardless of the kinetics and diffusional resistances. A means of calculating improved lower bounds is provided for cases where the cellular diffusional resistance is known but the kinetics are not. The optimal cell loadings for reversible first-order and for Michaelis-Menten kinetics are presented and demonstrated to be within the range of conditions of practical interest.

πŸ“œ SIMILAR VOLUMES

Optimization of the cell seeding density
Optimization of the cell seeding density and modeling of cell growth and metabolism using the modified Gompertz model for microencapsulated animal cell culture
✍ Qi Wen-tao; Zhang Ying; Ma Juan; Guo Xin; Xie Yu-bing; Wang Wei; Ma Xiaojun πŸ“‚ Article πŸ“… 2006 πŸ› John Wiley and Sons 🌐 English βš– 273 KB

## Abstract Cell microencapsulation is one of the promising strategies for the in vitro production of proteins or in vivo delivery of therapeutic products. In order to design and fabricate the optimized microencapsulated cell system, the Gompertz model was applied and modified to describe the growt

Specific ATP and specific oxygen uptake
Specific ATP and specific oxygen uptake rate in immobilized cell aggregates: Experimental results and theoretical analysis using a structured model of immobilized cell growth
✍ P. Gikas; A. G. Livingston πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 230 KB πŸ‘ 1 views

In this work the quality and activity of immobilized Beneckea natriegens have been measured using Specific ATP (SATP) [mg (ATP) g -1 (dry biomass)] and Specific Oxygen Uptake Rate (SOUR) [mg O 2 g -1 (dry biomass) h -1 ]. The cells were grown in a 3 L Three Phase Air Lift Bioreactor (TPALB) and were

A fast cell loading and high-throughput
A fast cell loading and high-throughput microfluidic system for long-term cell culture in zero-flow environments
✍ Chunxiong Luo; Xuejun Zhu; Tao Yu; Xianjia Luo; Qi Ouyang; Hang Ji; Yong Chen πŸ“‚ Article πŸ“… 2008 πŸ› John Wiley and Sons 🌐 English βš– 348 KB

## Abstract We present a simple technique for cell loading, culturing, and phenotypic study in a multi‐chamber microfluidic device made of polydimethylsiloxane (PDMS). This technique is based on the use of degassing induced aspiration of PDMS which allows loading cells into micro‐cavities within 1

Hollow fiber modules as a tool for enzym
Hollow fiber modules as a tool for enzyme and cell immobilization
✍ M. Rucka; Z. Sroka πŸ“‚ Article πŸ“… 1989 πŸ› John Wiley and Sons 🌐 English βš– 425 KB πŸ‘ 1 views

Owing to their properties, hollow fiber modules are attractive carriers for the immobilization of biocatalysts. Various systems and modes of operation are summarized and discussed.