The nondisjunction of artificial yeast minichromosomes (2:0 segregation events) during mitosis is accompanied by the appearance of cells containing more than one copy of the minichromosome. A mathematical simulation of this process has demonstrated that under certain conditions, a nondisjunction of
โฆ LIBER โฆ
A diagonal procedure for isolating sulfhydryl peptides alkylated with N-ethylmaleimide
โ Scribed by Heinz Gehring; Philipp Christen
- Book ID
- 102626918
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 260 KB
- Volume
- 107
- Category
- Article
- ISSN
- 0003-2697
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A simple three-step procedure is described for the purification of a labeled peptide from a tryptic digest of the P-subunit of the F,-ATPase after the enzyme had been inactivated with p-fluorosulfonyl-[14C]benzoyl-5'-adenosine. The procedure involves: (1) anion-exchange chromatography of a tryptic