A determination of the efficiency of uptake of SP50 DNA by competent cells of B. subtilis
β Scribed by Spatz, H. Ch.
- Publisher
- Springer
- Year
- 1972
- Tongue
- English
- Weight
- 226 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
At present there has been no satisfactory explanation of how such a large molecule as DNA can penetrate and transform a competent bacterium. A mechanism is proposed by which transforming DNA could be taken up by the mesosome organelles of Bacillus subtilis. The mesosomal vesicles could constitute an
The initial attachment of transforming DNA to competent Bacillus subtilis is temperature independent between 25 degrees and 45 degrees. However, below 15 degrees there is a significant reduction in the amount of DNA attached to compentent cells. The DNA that is attached at 4 degrees can lead to tran
The binding and uptake of nick-translated 32P-labeled pBR322 by Anacystis nidulans 6301 have been characterized. Both processes were considerably enhanced in permeaplasts compared to cells. The breakdown of labeled DNA was not correlated with binding or uptake by permeaplasts or cells. Uptake of DNA