A detailed study of the periodate oxidation of sialic acids in glycoproteins
โ Scribed by Gerd Reuter; Roland Schauer; Claudia Szeiki; Johannis P Kamerling; Johannes FG Vliegenthart
- Publisher
- Springer US
- Year
- 1989
- Tongue
- English
- Weight
- 533 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1573-4986
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๐ SIMILAR VOLUMES
Sialic acids were specifically labeled with o-phenylenediamine-2HCl (OPD) to yield stable fluorescent quinoxaline derivatives. The sialic acids were released from the glycoprotein in a NaHSO4 solution (0.25 M 80 degrees C, 20 min) and derivatized in the same solution with the OPD (10 mg/ml (final co
The peeling reaction, the process by which oligosaccharides are degraded in alkali, was used as the basis for an assay to provide structural information about glycosidically linked oligosaccharides in glycoproteins. Glycoproteins were treated with 0.05 M NaOH at 50 degrees to induce release, and sub