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A Defined System for in vitro Packaging of DNA--gp3 of the Bacillus subtilis Bacteriophage φ 29

✍ Scribed by Peixuan Guo, Shelley Grimes and Dwight Anderson


Book ID
123632869
Publisher
National Academy of Sciences
Year
1986
Tongue
English
Weight
904 KB
Volume
83
Category
Article
ISSN
0027-8424

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In vitro initiation of bacteriophage Φ29
✍ Matsumoto, Kouji ;Saito, Toshiyuki ;Hirokawa, Hideo 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 966 KB

Cell-free extracts prepared from phi 29 and M2-infected Bacillus subtilis cells catalyse the formation of complexes between terminal protein and [alpha-32P]-dAMP in the presence of [alpha-32P]-dATP, MgCl2, ATP, and phage DNA with terminal protein covalently linked at both the 5'ends. The complex for