𝔖 Bobbio Scriptorium
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A defective viral genome maintained in Escherichia coli for the generation of baculovirus expression vectors

✍ Scribed by Yeon Ho Je; Jin Hee Chang; Jae Young Choi; Jong Yul Roh; Byung Rae Jin; David R. O'Reilly; Seok Kwon Kang


Book ID
110288901
Publisher
Springer Netherlands
Year
2001
Tongue
English
Weight
105 KB
Volume
23
Category
Article
ISSN
0141-5492

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An intercompatible gene-tagging insert sequence was designed to conveniently introduce epitope-tagged polypeptides into bacteria and mammalian cells. The presence of rare restriction enzyme sites located between the ATG codon and the sequence encoding the introduced \(\boldsymbol{\epsilon}\)-tag cre