A decreased production of IL12 in vitro is associated with isolation of cytopathic HIV-1 strains in HIV-1-infected patients

✍ Scribed by Benyoucef, Samira; Hober, Didier; De Groote, Donat; Gérard, Yann; Lion, Georges; Bocket, Laurence; Mouton, Yves; Wattré, Pierre

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 93 KB
• Volume: 55
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/(sici)1096-9071(199807)55:3<209::aid-jmv5>3.0.co;2-5

✦ Synopsis

The changes in type 1 (IL12, IFN␥, IL2) and type 2 (IL4, IL10) cytokine profiles may be associated with virological parameters of progression of the disease in HIV-1-infected patients. The production of cytokines was studied in LPS + PHAactivated whole-blood culture in HIV-1-infected individuals at different stages of the disease. The association was investigated between IL12p40 and IL12p70 profiles and other cytokines (IFN␥, IL4, IL10), as well as the isolation of cytopathogenic HIV-1 strains. The phenotype of HIV strains was studied by a micromethod based on P4 cell line, allowing detection of cytopathic effects of HIV-1 isolates (syncytium-induction and cellkilling without syncytium induction). The individual variations in IL12p40 and IL12p70 production were limited in the healthy controls. Low values were observed in HIV-1-infected patients. The production of IL12 (p40 and p70) and the IL12p70/IL4 ratio and the IFN␥/IL4 ratio were significantly lower in patients with cytopathic isolates compared with patients with noncytopathic isolates, and a correlation was obtained between the values of IL12 (IL12p40 and IL12p70) and those of IFN␥/IL4 ratio. There was no increase in the secretion of IL4 and IL10 in patients with cytopathic strains compared with other patients. The results indicate a decreased production of type 1 cytokines (IL12, IFN␥) in the presence of a relatively preserved production of type 2 cytokines (IL4, IL10) in HIV-1-infected patients. In conclusion, the defect of production of IL12 by whole blood is associated with virological correlates of progression of HIV-1 disease.