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A cytokinin mutant derived from cultured tobacco cells

โœ Scribed by Meins, Frederick ;Foster, Rachel

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
414 KB
Volume
7
Category
Article
ISSN
0192-253X

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โœฆ Synopsis

Tissues cultured from the leaf lamina of wild-type Nicotiana tubacum L. cv. "Havana 425" plants require an exogenous source of cytokinin for rapid growth. In contrast, leaf tissues of plants heterozygous or homozygous for the partially dominant, monogenic habituated leaf (Hl-1) trait, exhibit a cytokinin-autotrophic phenotype in culture. Here we show that the H1 trait can arise in culture. Cytokinin autotrophic variants were obtained by culturing wild-type tissues of leaf lamina successively on media containing reduced concentrations of the cytokinin, kinetin. Plants regenerated from clones of these variants exhibited the H1 phenotype, which segregated in breeding tests as expected for a dominant, monogenic trait. This trait, designated H1-2, is inherited at a different locus than the H1-1 trait described earlier. Our results show that cytokinin mutants can arise in cell culture and that at least two genes regulate the cytokinin requirement of cultured tobacco tissues.

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