A cytochemical study of glycogen synthesized from glucose or pyruvate by liver slices in vitro
✍ Scribed by Deane, Helen Wendler ;Nesbett, Frances B. ;Buchanan, John M. ;Hastings, A. Baird
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1947
- Tongue
- English
- Weight
- 964 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0095-9898
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✦ Synopsis
NINE FIGURES
Ostern, Herbert and Holrnes ( '39) demonstrated by chemical means that slices of rabbit liver synthesized glycogen from glucose when incubated in a modified Ringer's solution, and Hastings and Buchanan ('42) found that glycogen was formed by slices of rat liver when incubated in media containing the intracellular cations potassium and magnesium but no sodium. However, neither the location of the new glycogen in the slices nor the morphological condition of the cells which permits such synthesis has been established. The present study concerns the histological distribution of the new glycogen in slices of rat and rabbit liver incubated in media containing either glucose or pyruvate, as well as observations on the nuclei, the mitochondria, fat and cytoplasmic basophilia.
MATERIAL AND METHODS
Four rats were fed for the same 2-hour period each day for 5 days and then killed by a blow on the head 24 hours after the last feeding. Four rabbits were killed in the same manner after a 48-hour starvation.
lThis inrestigation was done i n part under a grant to the Department of Anatomy from the Bmerican Cancer Society on the reeommendation of the Committee on Groivth of the National Research Council.