✦ LIBER ✦

A cycling assay for the determination of mono(adenosine-5′-diphosphoribose)-protein bound conjugate levels in mouse tissues

✍ Scribed by Chang He

Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 364 KB
Volume: 155
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(86)90245-9

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✦ Synopsis

Adenosine-5'-diphosphoribose (ADPR) is quantitatively split into 5'-AMP and ribose phosphate by treatment with alkali at elevated temperature. The 5'-AMP is used to generate NAD through a series of enzyme-catalyzed reactions. The NAD is then determined with a cycling assay modified after E.L. Jacobson and M.K. Jacobson [(1976) Arch. Biochem. Biophys. 175, 627-634]. The specificity of this assay has been verified. With this method the levels of mono(ADPR)-protein bound conjugate in various mouse tissues have been determined.