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A curve fitting method of quantifying green fluorescent protein expression level in Escherichia coli

✍ Scribed by Kun Zhang; Xuran Li; Yuanyuan Ma; Shaolan Zou; Lifeng Yang; Minhua Zhang


Book ID
116750801
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
396 KB
Volume
80
Category
Article
ISSN
0167-7012

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We have constructed three plasmid vectors for the expression of green fluorescent protein (GFP) fusion proteins using the following motif: (His) 6 -GFP-EK-X, where X represents chloramphenicol acetyl-transferase (CAT), human interleukin-2 (hIL-2), and organophosphorous hydrolase (OPH), respectively,