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A crude nuclease preparation suitable for use in DNA reassociation experiments

✍ Scribed by W.D. Sutton

Book ID
115744523
Publisher
Elsevier Science
Year
1971
Weight
602 KB
Volume
240
Category
Article
ISSN
0005-2787

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The optimal parameters in the use of nuclease S1 in DNA reassociation kinetics in the presence of formamide have been determined. The conditions are especially suitable for the study of DNA rich in mole percent GC. A 10-fold dilution of the reassociation samples leading to a decrease in both NaCl an