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A coupled spectrophotometric assay for trehalose 6-phosphate synthetase from Dictyostelium discoideum

✍ Scribed by Kathleen A. Killick

Book ID: 118564039
Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 668 KB
Volume: 1
Category: Article
ISSN: 0147-5975
DOI: 10.1016/s0147-5975(77)80020-0

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A specific and sensitive radiometric assay has been developed for the measurement of trehalose&phosphate (trehalose-6-P) synthetase activity. With either [\*T]glucose-6-P or UDP-['\*C]glucose as substrate, products unique to the synthetase reaction, i.e., trehalose-6-P and trehalose, may be quantita