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A Correlation between ovarian follicular maturity and anchorage-independent growth of bovine granulosa cells

✍ Scribed by Raphael K. Bartholomeusz; Ivan Bertoncello; Wayne A. Chamley


Book ID
112212166
Publisher
AlphaMed Press
Year
1988
Tongue
English
Weight
993 KB
Volume
6
Category
Article
ISSN
1066-5099

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## Abstract Normal, primary explanted, bovine granulosa cells grow reproducibly in agar culture as anchorage‐independent clones. Epidermal growth factor (EGF) and rat erythrocytes are effective stimulators of colony formation, and when both are added to the culture medium at optimal concentrations,