A correlated biochemical and histochemical study of succinic dehydrogenase activity in the gastric mucosa of the rat and frog

✍ Scribed by Villarreal, Ram�n ;Burgos, Mario H.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1955
• Tongue: English
• Weight: 746 KB
• Volume: 46
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030460209

✦ Synopsis

n a t o m y , Harvard Medical h'chool, Boston, Massachusetts

NINE FIGURES

This study is coiicerricd with the possibility of a direct correlation between the secretory functions of the gastric mucosa, and the metabolic activity of its cellular constituents. Since succinic dehydrogenase has been found to be concentrated in the gastric parietal cells (Padykula, ' 5 2 ) , it was possible to investigate whether there is any relationship between enzyme activity and the secretion of hydrochloric acid by these cells. Parallel histochemical and quantitative chemical studies were made on gastric mucosa of both rats and frogs in which acid secretion had been stimulated as well as upon normal controls. Acetyl-beta-methplcholine ( AIccholyl) was used in rats to stimulate the gastric secretion, whereas sodium salyrganate (Mersalyl) was used to inhibit it. Hydrochloric acid secretion was stiniulated in frogs by histamine, a drug to which the frog, but not the rat, is particularly sensitive.

Rats.

MATERIAL AND METHODS

Albino rats of the Sprague-Dawley strain, weighing 200 t o 250 gm, were fasted for 18 to 24 hours before use.