A convenient method of applying samples to thin layers, and of estimating phospholipids eluted from chromatoplates

Blotting of Glycolipids and Phospholipid

Blotting of Glycolipids and Phospholipids from a High-Performance Thin-Layer Chromatogram to a Polyvinylidene Difluoride Membrane

✍ T. Taki; S. Handa; D. Ishikawa 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 468 KB

A simple method of blotting glycosphingolipids from a high-performance thin-layer chromatography (HPTLC) plate to a polyvinylidene difluoride (PVDF) membrane is described. The developed HPTLC plate is dipped in a solvent mixture (isopropanol/0.2\% \(\mathbf{C a C l}_{2}\) / methanol, \(40 / 20 / 7\)