A convenient method for the preparation of a variety of 13C-substituted d-fructose phosphates using readily available enzymes of the glycolytic pathway

✍ Scribed by Kah T. Kuan; Denise S. Weber; Laura Sottile; Warren J. Goux

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 994 KB
• Volume: 225
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(92)80044-2

✦ Synopsis

Methods are presented for the preparation of a variety of D-fructose phosphates, 13C-substituted at any single carbon site or at any two symmetrically disposed carbon sites, from either 13C-substituted pyruvate or L-alanine. It is demonstrated that millimole quantities of product can be obtained in good yield following a "one-pot" incubation of 13C-substituted precursors with commercially available enzymes and cofactors of the glycolytic pathway. Since it has previously been shown that a wide variety of aldehydes serve as acceptable substrates for the final rabbit muscle aldolase-catalyzed condensation step, the method can potentially be applied to prepare a wide variety of 13C-substituted sugars and sugar phosphates.