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A convenient method for the construction and expression of GPI-anchored proteins

โœ Scribed by Harrison, Patrick T.; Hutchinson, M.J.; Allen, Janet M.


Book ID
126655761
Publisher
Oxford University Press
Year
1994
Tongue
English
Weight
175 KB
Volume
22
Category
Article
ISSN
0305-1048

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The N-linked glycans of recombinant leishmanolysin (GP63) expressed as a glycosylphosphatidylinositol (GPI)-anchored membrane protein or modified for secretion in Chinese hamster ovary (CHO) cells were analyzed by fast atom bombardment-mass spectrometry (FAB-MS). The glycans isolated from both membr